The positive effect that lactoferrin has on human immune status has been covered in previous articles (Lactoferrin and the innate immune response, Lactoferrin and virus defense). This effect is based on its antioxidant and antimicrobial properties. Lactoferrin’s role as both a direct and indirect antioxidant will be discussed below.

Free radicals occur in the body due to a wide variety of metabolic processes and are usually quickly and effectively neutralised.  Free radicals also arise as a result of physical exertion, stress or the influence of environmental factors such as cigarette smoke, environmental toxins or UV radiation. Immune cells also produce free radicals as part of targeted infection responses.

When these situations are resolved, the free radicals are eliminated.   If too many free radicals are formed, or persist for too long, or if there are problems neutralising them, they can attack and damage cells and  tissues. This is referred to as oxidative stress.

Over the long term, this can lead to premature aging, or to a wide variety of illnesses and chronic inflammation [1]. Ultimately, there is hardly any disease which doesn’t involve oxidative stress and damage from Reactive Oxygen Species (ROS) [2-4].

Certain metals, iron in particular, play a central role in the formation of free radicals.  Free iron offers a potential opportunity for the increased formation of free radicals, particularly in cell walls [5].

This reaction sequence is the iron-catalysed Haber-Weiss reaction.  The relatively harmless oxygen products, superoxide and hydrogen peroxide, are converted into the highly reactive hydroxyl radical. Lactoferrin has the ability to counteract the Haber-Weiss reaction [6].

Lactoferrin has the potential to act as an antioxidant at many levels.

In what’s known as the Fenton reaction, the 2nd stage of the Haber-Weiss reaction, divalent iron is converted into trivalent iron. This produces large amounts of free radicals that can trigger programmed cell death (apoptosis). The presence of an iron chelator, an iron-binding substance, can down-regulate this process.

By binding catalytic iron in body fluids and inflamed areas, lactoferrin counteracts iron-induced oxidative stress, thus protecting the cells from damage or cell death. (You can read more in our article on the role of lactoferrin & disorders of iron metabolism).

Lactoferrin can also act indirectly by binding bacterial cell components and toxins.  This reduces the need for the antibacterial activity of immune cells (macrophages), which is also a significant source of free radicals.

In a smaller study by Mulder et al. from 2008, the effects of oral lactoferrin administration on immunological and antioxidant parameters were investigated [7]. Eight healthy male subjects were first given a placebo over a period of three weeks.  This was then followed by lactoferrin at two different doses (1 week: 100mg, 1 week: 200 mg).  With reference to the antioxidant capacity, there was a significant improvement after the two weeks. The oxygen radical absorbance capacity in the blood served as the measurement parameter here.

Given the low number of participants, the results of this study would require confirmation in larger, randomised trials.  The authors conclude that in illnesses where the aetiology features oxidative stress and inflammation, oral administration of lactoferrin could play a supporting role.

1        Eid R, Arab NTT, Greenwood MT. Iron mediated toxicity and programmed cell death: A review and a re-examination of existing paradigms. Biochimica et biophysica acta. Molecular cell research 2017; 1864: 399–430.

2        Valko M, Jomova K, Rhodes CJ, Kuča K, Musílek K. Redox- and non-redox-metal-induced formation of free radicals and their role in human disease. Archives of toxicology 2016; 90: 1–37.

3        Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative Stress and Neurodegenerative Diseases: A Review of Upstream and Downstream Antioxidant Therapeutic Options. Current Neuropharmacology 2009; 7: 65–74.

4        Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D, Abete P. Oxidative stress, aging, and diseases. Clinical interventions in aging 2018; 13: 757–772.

5        Belizi S, Nazarova IA, Klimova IA, Prokof’ev VN, Pushkina NV. Antioxidant properties of lactoferrin from human milk. Bull Exp Biol Med 1999; 127: 471–473.

6        Britigan BE, Serody JS, Cohen MS. The role of lactoferrin as an anti-inflammatory molecule. Advances in experimental medicine and biology 1994; 357: 143–156.

7        Mulder AM, Connellan PA, Oliver CJ, Morris CA, Stevenson LM. Bovine lactoferrin supplementation supports immune and antioxidant status in healthy human males. Nutrition research (New York, N.Y.) 2008; 28: 583–589.